Journal of Multidisciplinary Applied Natural Science
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https://doi.org/10.47352/jmans.2774-3047.314
Rudy T M Situmeang
Sumardi Sumardi
Syangap Diningrat Sitompul
Aniska Legia
Tri Umiana Soleha
Ni Luh Gede Ratna Juliasih
Jarnuzi Ganlazuardi
The search for environmentally friendly antibacterial materials with excellent performance that does not make bacteria resistant is still a challenge. In this research, S/CuO/GO nanomaterials were prepared by the sol-gel method using pectin as an emulsifying agent. After freeze-drying and calcining processes, the prepared nanomaterials were characterized using XRD, UV-vis diffuse reflectance, FTIR, SEM-EDX-mapping, and TEM. The Gram-positive and Gram-negative antibacterial tests were done using the MIC and Kirby-Bauer disc diffusion methods. The characterization data showed that the antibacterial material has met the nano-size of 16 nm, and its antibacterial tests using the MIC method gave promising results. Furthermore, the best MIC test results for Escherichia coli and Bacillus sp. bacteria were obtained at a concentration of 0.01000 and 0.00125 mg/mL, respectively. In addition, the Kirby-Bauer disc diffusion antibacterial testing for E. coli and B. subtilis produced a clear zone of 12 and 18 mm with an exposure time of 15 and 30 min, respectively. These results proved that S/CuO/nGO is potent for antibacterial application.
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