Investigating the Antioxidant, Antimicrobial and Sensory Effects of Honey Propolis Extract as a Natural Preservative in Beef Burgers

Authors

DOI:

https://doi.org/10.47352/bioactivities.2963-654X.335

Keywords:

antioxidant, beef burger, propolis extract

Abstract

In this study, the antioxidant, antimicrobial and sensory effects of honey propolis extract as a natural preservative in beef burgers were investigated. First, propolis extract was produced at different percentages (0.5, 1.0, and 2.0%), and the control treatment, containing 0% propolis extract, was produced with a beef burger. The pH, antioxidant 2,2-diphenyl-1-picrylhydrazyl (DPPH), thiobarbituric acid (TBA), total volatile base nitrogen (TVB-N), and microbial characteristics (total microbial count, Escherichia coli, Staphylococcus aureus, mold, and yeast) and sensory characteristics (color, smell, texture and overall acceptance) during the storage period were subsequently investigated over a period of 8 days (0, 4, and 8). The results of the evaluation of different properties revealed that adding propolis extract to hamburger samples caused a significant decrease in pH, TBA, TVN, and microbial properties (total microbial count, E. coli, S. aureus, mold, and yeast) (p < 0.05). On the other hand, the amount of antioxidant property and sensory evaluation (color, smell, texture, and overall acceptance) also increased with the addition of propolis extract (p < 0.05). Additionally, the duration of storage caused a significant increase in pH, TBA, TVN, and microbial characteristics (total microbial count, E. coli, S. aureus, and mold and yeast), and a reduction in antioxidant properties and sensory evaluation (color, smell, texture, and overall acceptability) were found in hamburger samples (p < 0.05). The use of 2% propolis extract improved the oxidation properties and maintained the sensory properties during 8 days of storage for hamburger. Therefore, the use of 2% propolis extract improves the antioxidant and antimicrobial properties of hamburgers during storage and is a superior treatment.

References

[1] K. J. Khorshidi, A. Karimnia, S. Gharaveisi, and H. Kioumarsi. (2008). "The Effect of Monensin and Supplemental Fat on Growth Performance, Blood Metabolites, and Commercial Productivity of Zel Lambs". Pakistan Journal of Biological Sciences. 11 : 2395-2400. 10.3923/pjbs.2008.2395.2400.

DOI: https://doi.org/10.3923/pjbs.2008.2395.2400

[2] H. Kioumarsi, Z. S. Yahaya, and A. W. Rahman. (2012). "The Effect of Molasses/Mineral Blocks and Medicated Blocks on Performance, Efficiency, and Carcass Characteristics of Boer Goats". Annals of Biological Research. 3 : 4574-4577.

[3] H. Kioumarsi, M. Alidoust, and S. C. Allen. (2022). "Sustainable Development". Avaye Ostad.

[4] S. M. Mahdavian, F. Askari, H. Kioumarsi, R. Naseri Harsini, H. Dehghanzadeh, and B. Saboori. (2024). "Modeling the Linkage Between Climate Change, CH4 Emissions, and Land Use With Iran's Livestock Production: A Food Security Perspective". Natural Resources Forum.  10.1111/1477-8947.12532.

DOI: https://doi.org/10.1111/1477-8947.12532

[5] F. O. Areche, D. D. C. Flores, J. T. Huaman, N. Ruggerths, D. R. Vílchez, and M. A. Q. Solano. (2021). "Effect of Essential Oils of Rosmarinus officinalis and Petroselinum crispum on Artisanal Hamburgers, Huancavelica, Peru". Journal of Southwest Jiaotong University. 56 (6). 10.35741/issn.0258-2724.56.6.36.

DOI: https://doi.org/10.35741/issn.0258-2724.56.6.36

[6] I. H. Badar, Y. Li, H. Liu, Q. Chen, Q. Liu, and B. Kong. (2023). "Effect of Vegetable Oil Hydrogel Emulsion as a Fat Substitute on the Physicochemical Properties, Fatty Acid Profile, and Color Stability of Modified Atmosphere Packaged Buffalo Burgers". Meat Science. 199 : 109143. 10.1016/j.meatsci.2023.109143.

DOI: https://doi.org/10.1016/j.meatsci.2023.109143

[7] A. S. M. Nejad, S. Shabani, M. Bayat, and S. E. Hosseini. (2014). "Antibacterial Effect of Garlic Aqueous Extract on Staphylococcus aureus in Hamburger". Jundishapur Journal of Microbiology. 7 (11). 10.5812/jjm.13134.

DOI: https://doi.org/10.5812/jjm.13134

[8] N. A. Khakpour, H. Shadmehri, H. Amrulloh, and H. Kioumarsi. (2023). "Antibacterial Effect of Juglans regia, Citrus sinensis, Vicia faba, and Urtica urens Extracts Under In Vitro Conditions". Bioactivities. 1 (2): 74-80. 10.47352/bioactivities.2963-654X.195.

DOI: https://doi.org/10.47352/bioactivities.2963-654X.195

[9] E. B. Özvural, Q. Huang, and M. L. Chikindas. (2016). "Comparison of Quality and Microbiological Characteristics of Hamburger Patties Enriched With Green Tea Extract Using Three Techniques: Direct Addition, Edible Coating, and Encapsulation". LWT - Food Science and Technology. 68 : 385-390. 10.1016/j.lwt.2015.12.036.

DOI: https://doi.org/10.1016/j.lwt.2015.12.036

[10] L. Zhang and X. Piao. (2023). "Use of Aromatic Plant-Derived Essential Oils in Meat and Derived Products: Phytochemical Compositions, Functional Properties, and Encapsulation". Food Bioscience. 102520. 10.1016/j.fbio.2023.102520.

DOI: https://doi.org/10.1016/j.fbio.2023.102520

[11] S. Hosseini Khabbazi, S. Mansouripour, and S. Saremnezhad. (2023). "The Effect of Propolis Extract as a Valuable Natural Additive on the Quality Characteristics of Toast Bread". Food Science & Nutrition.  10.1002/fsn3.3500.

DOI: https://doi.org/10.1002/fsn3.3500

[12] S. M. H. Ghadiri Amrei, M. Ahmadi, S. A. Shahidi, P. Ariaii, and L. Golestan. (2023). "Study of the Physicochemical, Microbial, and Sensory Properties of Hamburgers Enriched With Turmeric and Omega-3 Loaded Nanoliposomes". Iranian Journal of Food Science and Industry.  

[13] A. Mozafari, A. Anvar, A. Mirzaei, and M. Ataee. (2023). "Analyzing Rosmarinus officinalis Essential Oil and Its Nanoemulsion on Beef Burger Shelf Life at Refrigerator Temperature". Journal of Food and Bioprocess Engineering. 6 (1): 69-80.

[14] H. Zhang, Y. Liang, X. Li, and H. Kang. (2020). "Effect of Chitosan-Gelatin Coating Containing Nanoencapsulated Tarragon Essential Oil on the Preservation of Pork Slices". Meat Science. 166 : 108137. 10.1016/j.meatsci.2020.108137.

DOI: https://doi.org/10.1016/j.meatsci.2020.108137

[15] N. Rezaei Savadkouhi, P. Ariaii, and M. Charmchian Langerodi. (2020). "Effect of Encapsulated Hyssop (Hyssopus officinalis L.) Extract in Biopolymer Nanoemulsions on Oxidative Stability of Soybean Oil". Food Science & Nutrition. 8 (2): 1264-1271. 10.1002/fsn3.1415.

DOI: https://doi.org/10.1002/fsn3.1415

[16] P. Ebrahimi, A. Lante, R. M. Scapin, S. Zannoni, B. Contiero, P. Catellani, and V. Giaccone. (2022). "Evaluation of Quality and Safety of Beef Hamburgers Fortified With Ozonated Extra Virgin Olive Oil". LWT - Food Science and Technology. 170 : 114100. 10.1016/j.lwt.2022.114100.

DOI: https://doi.org/10.1016/j.lwt.2022.114100

[17] Z. Ghorbani, N. Zamin Dar, M. Jalvan, and M. Golabadi. (2020). "Effect of Lal-Kohestan Essence on the Microbial Characteristics of Hamburger". Food Hygiene. 10 (2): 77-93.

[18] S. Iranian Institute of and R. Industrial. (2016). "Frozen Raw Hamburger - Characteristics and Test Methods," Iranian Institute of Standards and Industrial Research. 2304.

[19] L. Wu, I. Tsai, Y. Ho, Y. Hang, C. Lin, M. Tsai, and F. Mi. (2021). "Active and Intelligent Gellan Gum-Based Packaging Films for Controlling Anthocyanin Release and Monitoring Food Freshness". Carbohydrate Polymers. 254 : 117410. 10.1016/j.carbpol.2020.117410.

DOI: https://doi.org/10.1016/j.carbpol.2020.117410

[20] I. O. Korkmaz, C. Bilici, and S. Korkmaz. (2021). "Sensory, pH, Syneresis, Water-Holding Capacity, and Microbiological Changes in Homemade Yogurt Prepared With Maca (Lepidium meyenii) Powder and Propolis Extract". International Journal of Gastronomy and Food Science. 23 : 100291. 10.1016/j.ijgfs.2020.100291.

DOI: https://doi.org/10.1016/j.ijgfs.2020.100291

[21] L. Angiolillo, M. A. Del Nobile, and A. Conte. (2015). "The Extraction of Bioactive Compounds From Food Residues Using Microwaves". Current Opinion in Food Science. 5 : 93-98. 10.1016/j.cofs.2015.10.001.

DOI: https://doi.org/10.1016/j.cofs.2015.10.001

[22] M. Parcheta, R. Świsłocka, S. Orzechowska, M. Akimowicz, R. Choińska, and W. Lewandowski. (2021). "Recent Developments in Effective Antioxidants: The Structure and Antioxidant Properties". Materials. 14 (8):  10.3390/ma14081984.

DOI: https://doi.org/10.3390/ma14081984

[23] S. M. Cottica, H. Sabik, D. Bélanger, H. J. Giroux, J. V. Visentainer, and M. Britten. (2015). "Use of Propolis Extracts as Antioxidants in Dairy Beverages Enriched With Conjugated Linoleic Acid". European Food Research and Technology. 241 : 543-551. 10.1007/s00217-015-2483-1.

DOI: https://doi.org/10.1007/s00217-015-2483-1

[24] M. Hu and C. Jacobsen. (2016). "Oxidative Stability and Shelf Life of Foods Containing Oils and Fats". AOCS Press. 10.1016/B978-1-63067-056-6.00008-2.

DOI: https://doi.org/10.1016/B978-1-63067-056-6.00008-2

[25] M. Shabani, M. Mokhtarian, and R. Kazempour. (2021). "Investigation of the Possibility of Increasing the Oxidative Stability of Fish Hamburger Using Ethanolic Propolis Extract During Storage". Iranian Journal of Nutrition Sciences and Food Industries. 16 (1): 109-122. 10.52547/nsft.16.1.109.

DOI: https://doi.org/10.52547/nsft.16.1.109

[26] R. Vargas-Sánchez, G. Torrescano-Urrutia, B. Torres-Martínez, M. Pateiro, J. M. Lorenzo, and A. Sánchez-Escalante. (2019). "Propolis Extract as an Antioxidant to Improve Oxidative Stability of Fresh Patties During Refrigerated Storage". Foods. 8 : 614. 10.3390/foods8120614.

DOI: https://doi.org/10.3390/foods8120614

[27] M. Shabani, M. Mokhtarian, A. Kalbasi‐Ashtari, and R. Kazempoor. (2021). "Effects of extracted propolis ( Apis mellifera ) on physicochemical and microbial properties of rainbow‐trout fish burger patties". Journal of Food Processing and Preservation. 45 (12). 10.1111/jfpp.16027.

DOI: https://doi.org/10.1111/jfpp.16027

[28] R. Moghimi, A. Aliahmadi, D. J. McClements, and H. Rafati. (2016). "Investigations of the Effectiveness of Nanoemulsions From Sage Oil as Antibacterial Agents on Some Foodborne Pathogens". LWT - Food Science and Technology. 71 : 69-76. 10.1016/j.lwt.2016.03.018.

DOI: https://doi.org/10.1016/j.lwt.2016.03.018

[29] M. S. Santos, L. M. Estevinho, C. A. L. de Carvalho, A. L. da Silva Conceição, and R. C. de Castro Almeida. (2020). "Rheological and Sensorial Evaluation of Yogurt Incorporated With Red Propolis". Journal of Food Science and Technology. 57 (3): 1080-1089. 10.1007/s13197-019-04142-5.

DOI: https://doi.org/10.1007/s13197-019-04142-5

[30] C. Jansen-Alves, F. D. Krumreich, G. P. Zandoná, M. A. Gularte, C. D. Borges, and R. C. Zambiazi. (2019). "Production of Propolis Extract Microparticles With Concentrated Pea Protein for Application in Food". Food and Bioprocess Technology. 12 (5): 729-740. 10.1007/s11947-019-2246-2.

DOI: https://doi.org/10.1007/s11947-019-2246-2

[31] K. Pobiega, K. Kraśniewska, and M. Gniewosz. (2019). "Application of Propolis in Antimicrobial and Antioxidative Protection of Food Quality: A Review". Trends in Food Science & Technology. 83 : 53-62. 10.1016/j.tifs.2018.11.007.

DOI: https://doi.org/10.1016/j.tifs.2018.11.007

[32] C. Amadio, S. Farrando, and M. Zimmermann. (2019). "Effect of Chitosan Coating Enriched With Oregano Essential Oil on the Quality of Refrigerated Meat Hamburgers". Revista de la Facultad de Ciencias Agrarias. 51 : 173-189.

[33] B. A. Middleton. (2011). In: " B. LePage (Ed.) Wetlands" Springer. 105-120. 10.1007/978-94-007-0551-7_7.

DOI: https://doi.org/10.1007/978-94-007-0551-7_7

[34] L. Kalavari, N. Nasiri, F. Ahmadian, and H. Kioumarsi. (2022). "Enrichment of Doogh with Olive Leaf Extract and Investigation of Its Physicochemical, Microbial, and Sensory Properties during Storage at Room Temperature and Refrigerator". Journal of Multidisciplinary Applied Natural Science. 3 (1): 34-42. 10.47352/jmans.2774-3047.143.

DOI: https://doi.org/10.47352/jmans.2774-3047.143

[35] M. A. Rosen, H. Kioumarsi, and H. Gholipour Fereidouni. (2025). "Climate action and net-zero emissions". European Journal of Sustainable Development Research. 9 (4).  10.29333/ejosdr/16864.

DOI: https://doi.org/10.29333/ejosdr/16864

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Published

2025-12-29

How to Cite

Fakhraei, F., Nia, A. M., Ziabari, F. Z., & Kioumarsi, H. (2025). Investigating the Antioxidant, Antimicrobial and Sensory Effects of Honey Propolis Extract as a Natural Preservative in Beef Burgers. Bioactivities, 3(2), 72–87. https://doi.org/10.47352/bioactivities.2963-654X.335