Journal of Multidisciplinary Applied Natural Science
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2.1
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https://doi.org/10.47352/jmans.2774-3047.442
Imam Tazi
Wildan Panji Tresna
Wiwis Sasmitaninghidayah
Khairut Tamimi
Muthmainnah Muthmainnah
Atikah Qonitah
Lia Aprilia
Rapid and reliable identification of gelatin origin is critically important for food authentication and halal verification. This study proposes a label-free optical sensing approach based on surface plasmon resonance (SPR) for discriminating bovine, porcine, and fish gelatin. Gelatin solutions (1–8% w/v) were analyzed using a prism-coupled attenuated total reflection (ATR)-SPR configuration, where resonance angle shifts were extracted from reflectance minima. Complementary finite-difference time-domain (FDTD) simulations were performed to validate experimental trends and elucidate field interactions at the metal–dielectric interface. Results show distinct refractive index (RI) profiles and concentration-dependent resonance shifts for each gelatin type, enabling clear differentiation without chemical labeling. At higher concentrations (7–8%), fish gelatin exhibits the highest RI, followed by porcine and bovine gelatin. Porcine gelatin demonstrates the most linear and sensitive response, with a strong correlation (R² = 0.9412) between concentration and resonance angle shift. Simulation results are in good agreement with experimental data, confirming the sensitivity of SPR to subtle RI variations. The novelty of this work lies in the integration of ATR-SPR and numerical modeling for rapid, non-destructive, and label-free identification of gelatin sources across multiple animal origins. This approach offers a promising platform for real-time food authentication, quality control, and halal assurance, with potential for portable and on-site sensing applications.
[1] R. Peltomaa, B. Glahn-Martínez, E. Benito-Peña, and M. C. Moreno-Bondi. (2018). "Optical Biosensors for Label-Free Detection of Small Molecules". Sensors. 18 (12): 4126. 10.3390/s18124126.
DOI: https://doi.org/10.3390/s18124126[2] S. Primrose, M. Woolfe, and S. Rollinson. (2010). "Food Forensics: Methods for Determining the Authenticity of Foodstuffs". Trends in Food Science and Technology. 21 (12): 582-590. 10.1016/j.tifs.2010.09.006.
DOI: https://doi.org/10.1016/j.tifs.2010.09.006[3] M. Yashini, S. Shanmugasundaram, and C. K. Sunil. (2024). In: "Biosensors for Foodborne Pathogens Detection". Academic Press. 195-221. 10.1016/B978-0-323-95586-7.00009-5.
DOI: https://doi.org/10.1016/B978-0-323-95586-7.00009-5[4] X. Huang, L. Xia, and G. Li. (2023). "Recent Progress of Molecularly Imprinted Optical Sensors". Chemosensors. 11 (3): 168. 10.3390/chemosensors11030168.
DOI: https://doi.org/10.3390/chemosensors11030168[5] D. I. Ellis, V. L. Brewster, W. B. Dunn, J. W. Allwood, A. P. Golovanov, and R. Goodacre. (2012). "Fingerprinting Food: Current Technologies for the Detection of Food Adulteration and Contamination". Chemical Society Reviews. 41 : 5706-5727. 10.1039/c2cs35138b.
DOI: https://doi.org/10.1039/c2cs35138b[6] D. M. Hashim, Y. B. Che Man, R. Norakasha, M. Shuhaimi, Y. Salmah, and Z. A. Syahariza. (2010). "Potential Use of Fourier Transform Infrared Spectroscopy for Differentiation of Bovine and Porcine Gelatins". Food Chemistry. 118 (3): 856-860. 10.1016/j.foodchem.2009.05.049.
DOI: https://doi.org/10.1016/j.foodchem.2009.05.049[7] A. A. Karim and R. Bhat. (2009). "Gelatin Alternatives for the Food Industry: Recent Developments, Challenges and Prospects". Trends in Food Science and Technology. 20 (12): 644-656. 10.1016/j.tifs.2009.08.001.
DOI: https://doi.org/10.1016/j.tifs.2008.08.001[8] M. E. Ali, U. Hashim, S. Mustafa, and Y. B. Che Man. (2012). "Swine-Specific PCR-ELISA for Halal Authentication of Gelatin and Gelatin-Based Products". Food Control. 28 (1): 131-137. 10.1016/j.foodcont.2012.04.030.
DOI: https://doi.org/10.1016/j.foodcont.2012.04.030[9] A. Rohman and Y. B. Che Man. (2012). "Authentication of Halal Foods: A Review". Food Reviews International. 28 (1): 97-112. 10.1080/87559129.2011.595862.
DOI: https://doi.org/10.1080/87559129.2011.595862[10] J. Homola. (2008). "Surface Plasmon Resonance Sensors for Detection of Chemical and Biological Species". Chemical Reviews. 108 (2): 462-493. 10.1021/cr068107d.
DOI: https://doi.org/10.1021/cr068107d[11] M. R. Islam, A. N. M. Iftekher, K. R. Hasan, M. J. Nayen, S. B. Islam, A. Hossain, Z. Mustafa, and T. Tahsin. (2021). "Design and Numerical Analysis of a Gold-Coated Photonic Crystal Fiber Sensor". Optical and Quantum Electronics. 53 : 112. 10.1007/s11082-021-02748-8.
DOI: https://doi.org/10.1007/s11082-021-02748-8[12] M. H. Fuad, M. F. Nayan, and R. R. Mahmud. (2025). "Advances in Surface Plasmon Resonance Based PCF and MIM Sensors". Plasmonics. 20 : 7995-8026. 10.1007/s11468-025-02796-w.
DOI: https://doi.org/10.1007/s11468-025-02796-w[13] M. F. Nayan, M. A. Raihan, T. Ahmed, M. H. Fuad, N. A. Zaman, and R. R. Mahmud. (2025). "High Sensitivity One Dimensional Photonic Crystal Sensor Design for Waterborne Bacteria Detection". Sensing and Imaging. 26 (6). 10.1007/s11220-024-00532-y.
DOI: https://doi.org/10.1007/s11220-024-00532-y[14] A. Venien, D. Levieux, and A. Levieux. (2013). "Differentiation of Porcine and Bovine Gelatins by Surface Plasmon Resonance Imaging". Food Chemistry. 136 (3-4): 1489-1495. 10.1016/j.foodchem.2012.09.099.
DOI: https://doi.org/10.1016/j.foodchem.2012.09.099[15] M. Piliarik and J. Homola. (2009). "Surface Plasmon Resonance (SPR) Sensors: Approaching Their Limits?". Optics Express. 17 (19): 16505-16517. 10.1364/OE.17.016505.
DOI: https://doi.org/10.1364/OE.17.016505[16] W. Lu, L. Lin, and L. Jiang. (2006). "Nanogold Hollow Balls with Dendritic Surface for Hybridization of DNA". Biosensors and Bioelectronics. 22 (6): 1101-1105. 10.1016/j.bios.2006.03.024.
DOI: https://doi.org/10.1016/j.bios.2006.03.024[17] W. P. Tresna and T. Maruyama. (2019). "Propagation Loss on a Si-Slab Waveguide: Simulation Revisited". Journal of Physics and Its Applications. 2 (1): 76-78. 10.14710/jpa.v2i1.6421.
DOI: https://doi.org/10.14710/jpa.v2i1.6421[18] H. R. Gwon and S. H. Lee. (2010). "Spectral and Angular Responses of Surface Plasmon Resonance Based on the Kretschmann Prism Configuration". Materials Transactions. 51 (6): 1150-1155. 10.2320/matertrans.M2010003.
DOI: https://doi.org/10.2320/matertrans.M2010003[19] N. Hassan, T. Ahmad, and N. M. Zain. (2021). "Identification of Bovine, Porcine and Fish Gelatin Signatures Using Chemometrics Fuzzy Graph Method". Scientific Reports. 11 : 9793. 10.1038/s41598-021-89358-2.
DOI: https://doi.org/10.1038/s41598-021-89358-2[20] P. B. Johnson and R. W. Christy. (1972). "Optical Constants of the Noble Metals". Physical Review B. 6 : 4370-4379. 10.1103/PhysRevB.6.4370.
DOI: https://doi.org/10.1103/PhysRevB.6.4370[21] A. Qonitah, L. Aprilia, I. Darmadi, W. P. Tresna, and R. Nuryadi. (2023). "Observation of Glucose Concentration in Water Using Surface Plasmon Resonance". 2023 International Conference on Radar, Antenna, Microwave, Electronics, and Telecommunications (ICRAMET), Bandung, Indonesia. 226-230. 10.1109/ICRAMET60171.2023.10366707
DOI: https://doi.org/10.1109/ICRAMET60171.2023.10366707