Journal of Multidisciplinary Applied Natural Science
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4.8
Calculated on 05 May, 2025
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4.8
Calculated on 05 May, 2025
0.31
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https://doi.org/10.47352/jmans.2774-3047.333
Awalul Fatiqin
Rokiy Alfanaar
Sudarman Rahman
Yahya Febrianto
Shesanthi Citrariana
Mu’afa Purwa Arsana
Thathit Suprayogi
Yehezkiel Steven Kurniawan
Hanif Amrulloh
This study aims to synthesize and evaluate the antibacterial activity of silver nanoparticles (AgNPs) produced from the ethanolic extract of Drymoglossum piloselloides, highlighting its unique phytochemical composition rich in flavonoids, phenolics, and steroids that are expected to enhance nanoparticle stability and antibacterial performance. The methodology employed includes the synthesis of AgNPs through the reduction of leaf extract, followed by comprehensive characterization using UV-Vis spectroscopy, XRD, FTIR, TEM, and particle size analysis. The results indicate that AgNPs exhibit a spherical morphology with varying average sizes, with AgNPs 30 demonstrating the smallest size of 20.33 nm. The antibacterial activity of AgNPs was tested against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, producing inhibition zones up to 9.26 ± 1.02 mm against S. aureus. The proposed antibacterial mechanism involves the generation of reactive oxygen species and disruption of bacterial membranes, leading to oxidative stress and cell lysis. In conclusion, AgNPs synthesized from D. piloselloides show considerable potential as effective antibacterial agents, thereby opening opportunities for further applications in nanomedicine and infection control.
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