Journal of Multidisciplinary Applied Natural Science

Evaluation of The Anticancer Activity of Hydroxyxanthones Against Human Liver Carcinoma Cell Line

Yehezkiel Steven Kurniawan — 2023-05-23

Nowadays, cancer is one of the most fatal diseases in developed and developing countries. Therefore, it is an urgent need to find more effective anticancer drugs among the recent commercially available standard drugs. Xanthone derivatives have been researched as anticancer drugs due to their ease of synthesis and structure modification, as well as their excellent anticancer activity. In this work, the in vitro anticancer activity of hydroxyxanthones against the human liver carcinoma cell line (HepG2) was evaluated. Among the twenty-two hydroxyxanthones, 1,3,6,8-tetrahydroxyxanthone was found as the most active anticancer agent with an IC₅₀ value of 9.18 μM, which was better than doxorubicin as the standard drug. From the molecular docking studies against topoisomeraseIIα and two c-KIT protein kinases, 1,3,6,8-tetrahydroxyxanthone yielded strong binding energy in a range of -25.48 to -30.42 kJ/mol. The 1,3,6,8-tetrahydroxyxanthone could bind on the active site of these protein receptors through hydrogen bonds with key amino acid residues (Glu640, Cys673, Gln767, Met769, Asp810, and Asp831), as well as nitrogen bases (Adenine12 and Guanine13), thus leading to the death of HepG2 cancer cells through the apoptosis mechanism.

Cytotoxic Activity of Green Seaweed Halimeda tuna Methanolic Extract Against Lung Cancer Cells

Amir Husni — 2023-05-25

Lung cancer is a malignant tumor that attacks the lungs generated by carcinogenic free radicals such as cigarette smoke. Seaweed contains bioactive compounds that have the potential to reduce cancer-causing free radicals. This study aimed to determine the phytochemical content and cytotoxic activity of Halimeda tuna seaweed extract against lung cancer cells (A549). The H. tuna sample was macerated using methanol for 24 h. Cytotoxic test of H. tuna crude extract used the MTT test against A549. The crude extract was phytochemically tested and analyzed using gas chromatography–mass spectrometry (GC-MS). The results showed that the H. tuna crude extract had cytotoxic activity against A549 with an IC₅₀ value of 2771 µg/mL. The phytochemical test showed that H. tuna crude extract contained flavonoids and steroids. showed the presence of fatty acid compounds including palmitic acid, oleic acid, myristic acid, palmitoleic acid and stearic acid. Based on the results can be concluded that H. tuna extract had cytotoxic activity against A549 with low cytotoxicity to be used as a chemo-preventive agent.

Impact of Feed Point Position on Patch Antenna’s Return Loss and Bandwidth for UWB Applications

Md. Firoz Ahmed — 2023-06-15

The demand for compact, lightweight, and high-performance antennas has increased in recent times in the communication industry. Microstrip patch antenna (MPA) becomes a better choice to effectively fulfill these requirements. In this study, hybrid techniques of partial ground plane, slotted patch, and defective ground structure are employed in MPA design to reduce the return loss, good impedance matching, and increased the bandwidth, gain, and efficiency of the antenna. This research demonstrates the impact of altering the feed point position, a crucial phenomenon of antenna design, on the patch antenna and determines the proper feed point location by comparing a minimum return loss (S₁₁) which achieves the highest performance for the designed antenna. High-frequency structure simulator (HFSS) software is used to design and simulate the patch antenna. The operating frequency of the antenna is 6.85 GHz for UWB applications (3.1–10.6 GHz). A FR4 epoxy substrate material with dimensions of 30 mm × 20 mm is used to design the antenna. It has a dielectric constant of 4.4, a thickness of 0.8 mm and a tangent loss of 0.02. Multiple resonant frequencies are observed with different return losses for each feed location. The analysis shows that the finest feeding point is found at the center of the patch (9, 0) with a very low return loss (-28.35 dB), and a high impedance bandwidth (19.7 GHz). The antenna also achieved a gain of 4.46 dB, a directivity of 4.6904 dB, and a radiation efficiency of 95.90%. Hence, the location of the feed point can be considered as an influential factor in the antenna design.

The Utilization of Pectin as Natural Coagulant-Aid in Congo Red Dye Removal

Felicia M K Haryanto — 2023-06-21

Coagulation using inorganic compounds such as aluminum sulfate is commonly used in water-wastewater treatment. However, there are some drawbacks to its utilization, such as a significant decrease in the treated water’s pH, non-biodegradable sludge, and a potential negative impact on human mental health (dementia and Alzheimer's). The use of inorganic coagulants can be minimized with the addition of natural-based coagulant-aid such as pectin. In this study, Congo red solution, a model dye substance, was coagulated by varying the pH (3–7) using alum coagulant to determine the best pH for coagulation. At the best pH, pectin was introduced at various doses (0–30 mg/L), and subsequently various dye concentrations (50–100 mg/L). The effect of pectin as coagulant-aid was compared with aluminum sulfate and pectin only; with a response of %removal and sludge volume. It was found that the Congo red dye coagulation had the best %removal at pH 6 indicating a charge neutralization mechanism. The addition of 15 mg/L pectin at an aluminum sulfate dose of 30 mg/L resulted in 97.7% dye removal with a sludge volume of 14 mL/L at a Congo red concentration of 50 mg/L. This value is higher compared to those of aluminum sulfate and pectin only which gave 75.6 and 3.19% removals, respectively. Furthermore, the addition of pectin as a natural coagulant-aid could halve the sludge volume due to the formation of denser flocs. The results show a promising potential of pectin as a natural coagulant-aid in water-wastewater treatment.

Transesterification of Palm Oil Catalyzed by CaO/SiO2 Prepared from Limestone and Rice Husk Silica

Sri Elfina — 2023-07-14

In this study, CaO/SiO₂ composites were prepared from rice husk silica (RH-SiO₂) and limestone from a local company. The composites with different mass ratios of CaO to SiO₂ (1:1, 1:2, 1:3, 1:5, and 1:10) were synthesized using the sol-gel technique and characterized using XRF, XRD, and SEM. The composites were then used as catalysts for the transesterification of palm oil, with the main purpose to investigate the effect of catalyst compositions on the percentage of conversion of the oil. The results of XRD and SEM confirm the existence of RH-SiO₂ as an amorphous material, and CaO as crystalline material, while the composites are a mixture of amorphous and crystalline phases. The catalysts were then used in transesterification experiments and the percentage of oil conversion was calculated. To confirm the successful conversion of palm oil into fatty acid methyl esters, the products of the reactions were analyzed using GC-MS. The experimental results demonstrated that the composites prepared exhibit catalytic activity, with the highest conversion (60%) achieved using the catalyst with the CaO to SiO₂ ratio of 1:3.

Integration of Rational Functions

Laxmi Rathour — 2023-08-18

A rational function can always be integrated, that is, the integral of such a function is always an elementary function. The integration procedure is complex and consists of four steps: elimination of the common zero-points of the numerator and denominator, reduction to a true rational function, decomposition into partial fractions and integration of the obtained expressions using direct integration, substitution method or partial integration method. Integrating rational functions is important because integrals of rational functions of trigonometric functions as well as integrals of some irrational functions are reduced to integrals of rational functions by appropriate transformations.

Utilizing Satellite Imagery for Seasonal Trophic Analysis in the Freshwater Reservoir

Rifa Rachmadita — 2023-08-29

Eutrophication, an aquatic problem that impacts water quality and ecosystems, can be addressed through technological developments utilizing satellite imagery to analyze the trophic status of water. This study aimed to determine the trophic status of the freshwater reservoir in different seasons using satellite imagery. Water quality data were obtained from field surveys (11 samples) during the wet and dry seasons in Cirata Reservoir (Indonesia) while the remote sensing images were obtained from the Landsat-8 OLI. Trophic status refers to Carlson's Trophic State Index consisting of two parameters named chlorophyll-A and water transparency. This study found that satellite imagery could estimate seasonal trophic information. During the wet season, the algorithms determined information about chlorophyll-A (48%) and transparency (35%). Whereas during the dry season, the algorithms successfully estimated both information at 59% and 21%, respectively. Unfortunately, the Landsat-8 OLI had limitations for estimating total phosphorus. From these two parameters, the trophic status in the Cirata Reservoir showed moderate (wet season) and mild (dry season) eutrophic. Cirata Reservoir has a higher trophic level during the wet season since it has more surface water supply with lots of pollutants.

Influence of Water Table and Peat Thickness on Dissolved Organic Carbon of Tropical Peat Soil with Sulfidic Substratum from Central Kalimantan, Indonesia

Zafrullah Damanik — 2023-09-01

Peatlands are important due to their high carbon storage, their role in suppressing climate change processes, and their importance for local and global communities’ livelihood. Large amounts of organic carbon pools in peatlands can be released into the environment as gaseous emitted carbon and lost through waterways (fluvial). The carbon released through the water stream consists of organic and inorganic forms and is partly in the form of CO₂ and CH₄ gases. The organic form consists of dissolved organic carbon (DOC) and particulate organic carbon, where DOC is the most dominant organic carbon in water sourced from peatlands. This research's objectives were to study the DOC concentration of peat water resulting from the hydrological condition's difference and the peat thickness overlaying the sulfidic substratum. The study was carried out in the Pangkoh area of Pulang Pisau district of Central Kalimantan. Peat water is taken on PVC pipes installed on each plot representing different peat thicknesses (deep, moderate, and shallow peat) at a depth of 25, 50, 100, 150, 200, and 250 cm from the soil surface. The water sampling was conducted on the peak wet season, during the transition from wet season to dry season and during the peak dry season. The results showed that DOC was influenced by peat thickness, depth of sulfidic material, and groundwater level. The release of DOC is higher from the deep peat than from the thin and moderate peat. The difference in DOC concentration between peat thickness is also related to the electrical conductivity of the peat water. The results showed a negative correlation between electrical conductivity and DOC concentration. The negative correlation was significant in the observation of the rainy and dry seasons, while in the transitional season, it was not significant.

Fixed Point Theorems for Semigroups of Lipschitzian Mappings

Saiful Marom — 2023-09-27

The main purpose of this paper is to extend the results of fixed point theorems for lipschitzian semigroups. The proofs we give follow the results of Ishihara, Suantai and Puengrattana theorems. Using one of the proofs, we also develop a fixed point theorem result for nonempty asymptotically total mapping semigroups on uniformly convex Banach spaces.

Enhancing Photocatalytic Activity of Bismuth Ferrite (BiFeO3) via Gadolinium and Copper Doping: A Sol-Gel Synthesis and Characterization Study

Beerelli Rajitha — 2023-10-03

In this current research work, the sol-gel method was employed to synthesise, characterize and evaluate the photocatalytic activity of bismuth ferrite (BiFeO₃, BFO) doped with two distinctive components consisting of a rare earth element Gadolinium (Gd) and a transition metal Copper (Cu). The dopant concentrations were systematically varied with different weight percentages (wt.%) denoted as Bi_1-xGd_xFe_1-yCu_yO₃ (where ‘x’ = 0.10, 0.15 and 0.20 wt.%, where ‘y’ = 0.05, 0.10, and 0.15 wt.%). Subsequently, characterizations of the prepared samples were conducted using an array of cutting-edge analytical techniques including X-ray diffraction (XRD), filed emission scanning electron microscopy (FE-SEM), energy dispersive X-ray analysis (EDAX), and transmission electron microscopy (TEM). The XRD analysis results indicated that the presence of small impurity peaks was found in both Gd-doped BFO and GdCu-doped BFO. The FE-SEM and TEM results provided confirmation that the material was observed as a spherical shape, and the elemental compositions were also confirmed through EDAX analysis. The photocatalytic degradation of Rhodamine B dye under the influence of visible light irradiation was carried out and the results revealed varying degradation times, specifically, for Gd and Cu-doped BFO (Gd and Cu = 0.1 wt.%) achieved almost 98% degradation occurred in 30 minutes.

Advancing Green Nanotechnology: Harnessing the Bio-reducing Properties of Musa paradisiaca Peel Extract for Sustainable Synthesis of Iron Oxide Nanoparticles

Esther Uwidia Ikhuoria — 2023-10-07

A green synthesis method utilizing Musa paradisiaca peel extract as a reducing and stabilizing agent was employed to produce iron oxide nanoparticles. The synthesized nanoparticles were extensively characterized using FTIR, XRD, DLS, SEM, EDX, and TEM techniques. FTIR analysis confirmed the presence of iron metal and functional groups derived from the peel extract. XRD results indicated the presence of magnetite (Fe₃O₄) and/or maghemite (γ-Fe₂O₃) phases, signifying a high degree of crystallinity. DLS analysis provided valuable insights into the size distribution and polydispersity of the nanoparticles, revealing an average particle diameter of 43.35 nm and a polydispersity index of 0.612. SEM examination uncovered the presence of aggregated formations, where irregularly shaped nanoparticles exhibited either close packing or loose association, resulting in the formation of larger aggregates. These environmentally friendly iron oxide nanoparticles could potentially hold great promise for a variety of biological applications, including the potential for drug delivery and antimicrobial applications.

The Potential of Moringa Leaf Nanoparticles (Moringa oleifera) on the Expression of TNFα, IL10, and HSP 27 in Oral Cavity Cancer

Theresia Indah Budhy — 2023-11-22

Oral cancer is currently the sixth leading malignancy in the world, with over 330,000 cases resulting in death. Several cytokines and proteins protect the survival of cancer cells, such as TNF-α, HSP27, and IL-10. Moringa oleifera is an herbal medicine with anti-cancer properties. Nanoparticles of M. oleifera have the property to be easily absorbed by water-soluble cells, so only small doses are needed to be used as anti-cancer ingredients. This research aims to prove the ability of M. oleifera nanoparticle extract against oral cancer through the expression of tumour necrosis factor α (TNF-α), heat shock protein (HSP27), and interleukin 10 (IL-10). Rat as non-human experimental subjects were divided into four groups: control group (K), treatment group 1 (P1), treatment group 2 (P2), and treatment group 3 (P3). Cancer induction was carried out by injecting with benzo[a]pyrene, and then M. oleifera nanoparticle extract was administered in three forms of treatment doses of 125 μg/mL (P1), 250 μg/mL (P2), and 500 μg/mL (P3). Immunohistochemical examination was analysed through TNF-α, HSP27, and IL-10’s expression. The expression of TNF-α and HSP27 between control and treatment groups was significantly different. P2 had the lowest expression of TNF-α and HSP27. The expression of IL-10 between control and treatment groups was also significantly different. P1 had the lowest expression of IL-10. M. oleifera nanoparticle extract can reduce oral cancer progression by decreasing the expression of TNF-α, HSP27, and IL-10.

Evaluation of The Integrated Protocol for The Management of Severe Malnutrition in Children at The National Hospital of Nouakchott-Mauritania

Sima Boulebatt Mahfoud — 2023-12-09

Malnutrition is a worldwide concern with worrisome outcomes that affect substantially the lifestyle of infants. The main aim is to investigate the efficacy of the IMSAM protocol over malnourished Mauritanian children during hospitalization. A cohort of 230 participants, including 126 boys and 104 girls collected from rural and urban areas with an average age of 15.52 ± 7.727 months were enrolled. The study is carried out in the Pediatric Service of the National Hospital Center of Nouakchott, Mauritania on children who have undergone the national protocol for the Integrated Management of Severe Acute Malnutrition (IMSAM). In addition, the medical and nutritional treatment, and body measurements were appreciated as well as the anthropometric parameters. The findings exhibit during nutritional-based intervention significant differences between the mean of weight during the pre- and post-intervention (P < 0.001). Moreover, through a regression test, we found a negatively predicted weight gain (P < 0.05) by the variables Z-score of weight to height (P / T) Edema after Nutritional Support and final health status. Last, but by no means least, the IMSAM-based intervention with rational management might make inroads into mitigating adverse outcomes of malnutrition through maximizing social skills.

Antioxidant and Antibacterial Activities of Curd and Whey Kefir Produced from Etawa Goat Milk

Siwi Meutia Sadewi — 2023-12-11

Kefir is a fermented drink with many benefits. Kefir contains antioxidant compounds that can inhibit free radical activity.In addition, kefir contains lactic acid bacteria, which provide good antibacterial activity and improve the health of the digestive tract. This research aimed to determine the antioxidant and antibacterial activities of kefir made from Etawa goat milk. The methods used in this research included fermentation of Etawa goat milk with 10 % kefir seeds at 37 °C and a fermentation time of 2 days. Antioxidant activity was tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH), and antibacterial activity was tested using agar diffusion. The peptide molecular weights in kefir were analyzed using SDS-PAGE. The results showed that the antibacterial and antioxidant activities in Etawa goat's milk curd kefir has a higher activity than whey kefir. The antioxidant activity of curd has an IC₅₀ of 43.99 ppm, which is a powerful antioxidant. Curd's antibacterial activity forms a clear zone of 7 mm against Bacillus cereus and is included in the solid antibacterial category. In Escherichia coli, a clear zone of 6 mm was formed and included in the moderate antibacterial category. The results of SDS-PAGE gel visualization found three prominent bands measuring 10, 15, and 33 kDa, respectively.

Application of Response Surface Methodology (RSM) to Study Transesterification of Palm Oil in the Presence of Zeolite-A as Catalyst

Kamisah Delilawati Pandiangan — 2023-12-19

In this research, the application of response surface methodology with central composite design (RSM-CCD) to optimizetransesterification of palm oil in the presence of zeolite-A as catalyst was investigated. Zeolite-A was synthesized from rice husk silica (RHS) and food-grade aluminium foil using hydrothermal method and then characterized using XRD and SEM. The synthesized zeolite was then applied to an optimized transesterification reaction using response RSM with three factorial levels, for three variables including methanol to oil ratio, catalyst load, and reaction time. The experimental results indicate that the yield of 99% was achieved at optimum conditions of methanol to oil volume ratio of 6, catalyst load of 9.6%, and reaction time of 4.3 hours. The results of experiments and predicted results based on the RSM model are in agreement as shown by the p-value less than 0.05 at a confidence level of 95%.

Comparative Analysis Between Two Reliability Models of a Three-Unit Complex Industrial System

Syed Zegham Taj — 2024-01-20

A comparative analysis between two reliability models of a three-unit complex industrial system has been presented in this paper. Previously, real failure-maintenance data of a three-unit complex industrial system were collected. The situations depicted in the data were used for developing two appropriate reliability models for the system. Important reliability indicators of the system were estimated by applying Semi-Markov and regenerative processes. Here, a comparison between the reliability indices and profits of the two models is performed, which helps in identifying the suitability of one model over the other. Cut-off points for profits based on costs, revenues, and failure rates have also been obtained for deciding which of the two models is more profitable. Graphical analysis is also presented to illustrate the outcomes.

Characterization of Extracellular Chitinase from Bacillus cereus SAHA 12.13 and Its Potency as a Biocontrol of Curvularia affinis

Muhammad Asril — 2024-01-20

Bacillus cereus SAHA 12.13 can produce chitinase, an enzyme that digests chitin in the main compounds of cell walls, mycelia, and spores in pathogenic fungi that cause leaf spots on oil palm plants such as Curvularia affinis. This study aims to determine the properties of the chitinase enzyme B. cereus SAHA 12.13 that can inhibit the growth of C. affinis. Chitinase enzyme production and characterization were measured using the Spindler method. Antagonism test against pathogenic fungi using dual culture method by testing cell culture and enzyme crude extract. This result showed that the isolate produced a high level of specific chitinase activity at 37 °C for 45 h of incubation with 8.45 U mg^-1 proteins with a growth rate (k) of 0.25 generation/h, and the generation time was 3.96 h/generation. The optimum chitinase activity was achieved at pH 7.0 and 45 °C and was stable for 3 h with a half-life (t_1/2) of 770 min. The crude enzyme and cell culture of strain can inhibit the growth of C. affinis by 36.27±0.043% and 34.25±0.041%, respectively. These characteristics indicate that B. cereus strain SAHA12.13 can be used to inhibit C. affinis, which causes leaf blight of oil palm, under varying pH and temperature conditions.

Green Synthesis of ZnO Nanoparticles using Abelmoschus esculenthus L. Fruit Extract: Antioxidant, Photoprotective, Anti-inflammatory, and Antibacterial Studies

Christina Astutiningsih — 2024-01-30

Nanoparticles are extensively studied for their promising biological properties. In this study, the fruit extract of Abelmoschus esculenthus L. was used as a chelating agent for the synthesis of zinc oxide nanoparticles (ZnOPs-AE) using a zinc acetate solution. The prepared ZnOPs-AE were identified and characterized using UV-vis spectroscopy, Fourier-transformed infrared spectroscopy (FTIR), particle size analyzer (PSA), scanning electron microscopy (SEM), and energy dispersive spectrum (EDS). The green synthesized ZnOPs-AE were evaluated for their antioxidant, photoprotective, anti-inflammatory, and antibacterial activities. The synthesized nanoparticles showed an intensity peak at 370 nm in the UV-vis spectrum. The FTIR result shows the presence of O-H, C=O, C-O, C-OH, and C=C chelating functional groups on the surface of nanoparticles. The size of ZnOPs-AE was determined using a PSA with particle size distribution of 102.2 nm. The ZnOPs-AE were shown to be spherical by SEM analysis and composition was 82.11% and 14.79% for Zn and O, respectively. The antioxidant properties of ZnOPs-AE showed significant antioxidant potential in DPPH, ABTS, and FRAP assays compared to the quercetin standard. The photoprotection activity test showed a SPF value of 19.63, the percentage of erythema transmission was 5.98%, and the percentage of pigmentation transmission was 5.62%. The ZnOPs-AE showed good anti-inflammatory with the synthesized nanoparticle performing activity between positive control and the fruit extract of Abelmoschus esculenthus L. Also, the ZnOPs-AE exhibited good antibacterial activity against Staphylococcus aureus (20.78 mm) and Pseudomonas aeruginosae (11.13 mm). Overall, the results highlight the effectiveness and potential of ZnOPs-AE for biological application.

Chicken Eggshell Powder as Antibacterial Against Staphylococcus aureus and Escherichia coli Through In Vitro Studies

Rodhiansyah Djayasinga — 2024-01-31

Identifying the most effective material with antibacterial properties against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) is a challenging task considering the rising concerns about drug resistance. Various experiments through in vitro and in vivo studies to obtain antibacterial agents using abundant and easily available raw material sources have been conducted. Therefore, this study aimed to acquire semiconducting nanoparticle material derived from purebred chicken eggshell waste that could effectively function as an antibacterial agent. The waste treatment was carried out using a top-down method applying the thermal decomposition method with calcination temperatures of 700 and 800 ºC for 30 hours. XRD analysis results showed CaO as a major phase and this was further supported by Rietveld calculation. The size of the crystalline phases obtained ranged from 10–45 nm, while FTIR analysis showed the appearance of CaO bond at a wave number of 715.65 cm^-1. Furthermore, SEM analysis showed a rough folded particle surface with a pore percentage of 48.20%. Based on the UV-Vis DRS analysis results, chicken eggshell powder had band gap energy characteristics of 2.07, 2.74, 3.71, and 5.96 eV for sample B, as well as 4.60 and 5.82 eV for sample C. Activation of purebred chicken eggshell powder as antibacterial was performed both qualitatively and quantitatively using photocatalytic and non-photocatalytic methods. Qualitatively, both samples showed antibacterial activity, with a minimal inhibitory concentration (MIC) of 1,000 µg/mL.